rabbit anti-claudin-3 pab ( Search Results


c-myc antibody  (NSJ Bioreagents)
99
NSJ Bioreagents c-myc antibody
C Myc Antibody, supplied by NSJ Bioreagents, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/c-myc antibody/product/NSJ Bioreagents
Average 99 stars, based on 1 article reviews
c-myc antibody - by Bioz Stars, 2026-03
99/100 stars
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mouse anti-claudin-4 mab  (Thermo Fisher)
90
Thermo Fisher mouse anti-claudin-4 mab
Mouse Anti Claudin 4 Mab, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti-claudin-4 mab/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
mouse anti-claudin-4 mab - by Bioz Stars, 2026-03
90/100 stars
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rabbit anti–claudin-3 pab (#34-1700)  (Thermo Fisher)
90
Thermo Fisher rabbit anti–claudin-3 pab (#34-1700)
TJs of Tric -KO, Ocln -KO, and Tric / Ocln -dKO cells appear normal in fluorescence microscopy. (A–D) Ctrl, Tric -KO #1, Ocln -KO #1, or Tric / Ocln -dKO #1 cells were mixed and cocultured with Ctrl-GFP-nls cells (green). Cells were immunostained with rat anti-ZO-1 mAb (A), mouse anti-cingulin mAb (B), mouse anti–claudin-1 mAb (C) or rabbit <t>anti–claudin-3</t> pAb (D) (red) and stained with DAPI (blue). Scale bars, 20 µm. (E) Quantification of fluorescence intensity of claudin-3 at cell–cell junctions. The intensity at each cell–cell junction in the Ctrl (dark blue closed circles), Tric -KO #1 (orange closed circles), Ocln -KO #1 (green closed circles), and Tric / Ocln -dKO #1 cells (magenta closed circles) were normalized to the averaged intensity in the Ctrl-GFP-nls cells (lime-green open circles) and plotted against the length of the cell–cell junctions. n = 158 and 120 (Ctrl), 202 and 134 ( Tric -KO), 160 and 139 ( Ocln -KO), and 209 and 136 ( Tric / Ocln -dKO). *** p < 0.001 in Welch’s t test of junction-length-weighted average of the fluorescence intensity.
Rabbit Anti–Claudin 3 Pab (#34 1700), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti–claudin-3 pab (#34-1700)/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
rabbit anti–claudin-3 pab (#34-1700) - by Bioz Stars, 2026-03
90/100 stars
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claudin-3  (Thermo Fisher)
90
Thermo Fisher claudin-3
TJs of Tric -KO, Ocln -KO, and Tric / Ocln -dKO cells appear normal in fluorescence microscopy. (A–D) Ctrl, Tric -KO #1, Ocln -KO #1, or Tric / Ocln -dKO #1 cells were mixed and cocultured with Ctrl-GFP-nls cells (green). Cells were immunostained with rat anti-ZO-1 mAb (A), mouse anti-cingulin mAb (B), mouse anti–claudin-1 mAb (C) or rabbit <t>anti–claudin-3</t> pAb (D) (red) and stained with DAPI (blue). Scale bars, 20 µm. (E) Quantification of fluorescence intensity of claudin-3 at cell–cell junctions. The intensity at each cell–cell junction in the Ctrl (dark blue closed circles), Tric -KO #1 (orange closed circles), Ocln -KO #1 (green closed circles), and Tric / Ocln -dKO #1 cells (magenta closed circles) were normalized to the averaged intensity in the Ctrl-GFP-nls cells (lime-green open circles) and plotted against the length of the cell–cell junctions. n = 158 and 120 (Ctrl), 202 and 134 ( Tric -KO), 160 and 139 ( Ocln -KO), and 209 and 136 ( Tric / Ocln -dKO). *** p < 0.001 in Welch’s t test of junction-length-weighted average of the fluorescence intensity.
Claudin 3, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/claudin-3/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
claudin-3 - by Bioz Stars, 2026-03
90/100 stars
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claudin-3 rabbit polyclonal antibody rb-9251-p1  (Thermo Fisher)
90
Thermo Fisher claudin-3 rabbit polyclonal antibody rb-9251-p1
TJs of Tric -KO, Ocln -KO, and Tric / Ocln -dKO cells appear normal in fluorescence microscopy. (A–D) Ctrl, Tric -KO #1, Ocln -KO #1, or Tric / Ocln -dKO #1 cells were mixed and cocultured with Ctrl-GFP-nls cells (green). Cells were immunostained with rat anti-ZO-1 mAb (A), mouse anti-cingulin mAb (B), mouse anti–claudin-1 mAb (C) or rabbit <t>anti–claudin-3</t> pAb (D) (red) and stained with DAPI (blue). Scale bars, 20 µm. (E) Quantification of fluorescence intensity of claudin-3 at cell–cell junctions. The intensity at each cell–cell junction in the Ctrl (dark blue closed circles), Tric -KO #1 (orange closed circles), Ocln -KO #1 (green closed circles), and Tric / Ocln -dKO #1 cells (magenta closed circles) were normalized to the averaged intensity in the Ctrl-GFP-nls cells (lime-green open circles) and plotted against the length of the cell–cell junctions. n = 158 and 120 (Ctrl), 202 and 134 ( Tric -KO), 160 and 139 ( Ocln -KO), and 209 and 136 ( Tric / Ocln -dKO). *** p < 0.001 in Welch’s t test of junction-length-weighted average of the fluorescence intensity.
Claudin 3 Rabbit Polyclonal Antibody Rb 9251 P1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/claudin-3 rabbit polyclonal antibody rb-9251-p1/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
claudin-3 rabbit polyclonal antibody rb-9251-p1 - by Bioz Stars, 2026-03
90/100 stars
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rabbit anti-zo-2 pab  (Santa Cruz Biotechnology)
90
Santa Cruz Biotechnology rabbit anti-zo-2 pab
TJs of Tric -KO, Ocln -KO, and Tric / Ocln -dKO cells appear normal in fluorescence microscopy. (A–D) Ctrl, Tric -KO #1, Ocln -KO #1, or Tric / Ocln -dKO #1 cells were mixed and cocultured with Ctrl-GFP-nls cells (green). Cells were immunostained with rat anti-ZO-1 mAb (A), mouse anti-cingulin mAb (B), mouse anti–claudin-1 mAb (C) or rabbit <t>anti–claudin-3</t> pAb (D) (red) and stained with DAPI (blue). Scale bars, 20 µm. (E) Quantification of fluorescence intensity of claudin-3 at cell–cell junctions. The intensity at each cell–cell junction in the Ctrl (dark blue closed circles), Tric -KO #1 (orange closed circles), Ocln -KO #1 (green closed circles), and Tric / Ocln -dKO #1 cells (magenta closed circles) were normalized to the averaged intensity in the Ctrl-GFP-nls cells (lime-green open circles) and plotted against the length of the cell–cell junctions. n = 158 and 120 (Ctrl), 202 and 134 ( Tric -KO), 160 and 139 ( Ocln -KO), and 209 and 136 ( Tric / Ocln -dKO). *** p < 0.001 in Welch’s t test of junction-length-weighted average of the fluorescence intensity.
Rabbit Anti Zo 2 Pab, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti-zo-2 pab/product/Santa Cruz Biotechnology
Average 90 stars, based on 1 article reviews
rabbit anti-zo-2 pab - by Bioz Stars, 2026-03
90/100 stars
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rabbit anti–zo-2 pab  (Thermo Fisher)
90
Thermo Fisher rabbit anti–zo-2 pab
TJs of Tric -KO, Ocln -KO, and Tric / Ocln -dKO cells appear normal in fluorescence microscopy. (A–D) Ctrl, Tric -KO #1, Ocln -KO #1, or Tric / Ocln -dKO #1 cells were mixed and cocultured with Ctrl-GFP-nls cells (green). Cells were immunostained with rat anti-ZO-1 mAb (A), mouse anti-cingulin mAb (B), mouse anti–claudin-1 mAb (C) or rabbit <t>anti–claudin-3</t> pAb (D) (red) and stained with DAPI (blue). Scale bars, 20 µm. (E) Quantification of fluorescence intensity of claudin-3 at cell–cell junctions. The intensity at each cell–cell junction in the Ctrl (dark blue closed circles), Tric -KO #1 (orange closed circles), Ocln -KO #1 (green closed circles), and Tric / Ocln -dKO #1 cells (magenta closed circles) were normalized to the averaged intensity in the Ctrl-GFP-nls cells (lime-green open circles) and plotted against the length of the cell–cell junctions. n = 158 and 120 (Ctrl), 202 and 134 ( Tric -KO), 160 and 139 ( Ocln -KO), and 209 and 136 ( Tric / Ocln -dKO). *** p < 0.001 in Welch’s t test of junction-length-weighted average of the fluorescence intensity.
Rabbit Anti–Zo 2 Pab, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti–zo-2 pab/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
rabbit anti–zo-2 pab - by Bioz Stars, 2026-03
90/100 stars
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claudin  (Thermo Fisher)
86
Thermo Fisher claudin
TJs of Tric -KO, Ocln -KO, and Tric / Ocln -dKO cells appear normal in fluorescence microscopy. (A–D) Ctrl, Tric -KO #1, Ocln -KO #1, or Tric / Ocln -dKO #1 cells were mixed and cocultured with Ctrl-GFP-nls cells (green). Cells were immunostained with rat anti-ZO-1 mAb (A), mouse anti-cingulin mAb (B), mouse anti–claudin-1 mAb (C) or rabbit <t>anti–claudin-3</t> pAb (D) (red) and stained with DAPI (blue). Scale bars, 20 µm. (E) Quantification of fluorescence intensity of claudin-3 at cell–cell junctions. The intensity at each cell–cell junction in the Ctrl (dark blue closed circles), Tric -KO #1 (orange closed circles), Ocln -KO #1 (green closed circles), and Tric / Ocln -dKO #1 cells (magenta closed circles) were normalized to the averaged intensity in the Ctrl-GFP-nls cells (lime-green open circles) and plotted against the length of the cell–cell junctions. n = 158 and 120 (Ctrl), 202 and 134 ( Tric -KO), 160 and 139 ( Ocln -KO), and 209 and 136 ( Tric / Ocln -dKO). *** p < 0.001 in Welch’s t test of junction-length-weighted average of the fluorescence intensity.
Claudin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/claudin/product/Thermo Fisher
Average 86 stars, based on 1 article reviews
claudin - by Bioz Stars, 2026-03
86/100 stars
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rabbit anti–claudin-2 pab (#51-6100)  (Thermo Fisher)
90
Thermo Fisher rabbit anti–claudin-2 pab (#51-6100)
TJs of Tric -KO, Ocln -KO, and Tric / Ocln -dKO cells appear normal in fluorescence microscopy. (A–D) Ctrl, Tric -KO #1, Ocln -KO #1, or Tric / Ocln -dKO #1 cells were mixed and cocultured with Ctrl-GFP-nls cells (green). Cells were immunostained with rat anti-ZO-1 mAb (A), mouse anti-cingulin mAb (B), mouse anti–claudin-1 mAb (C) or rabbit <t>anti–claudin-3</t> pAb (D) (red) and stained with DAPI (blue). Scale bars, 20 µm. (E) Quantification of fluorescence intensity of claudin-3 at cell–cell junctions. The intensity at each cell–cell junction in the Ctrl (dark blue closed circles), Tric -KO #1 (orange closed circles), Ocln -KO #1 (green closed circles), and Tric / Ocln -dKO #1 cells (magenta closed circles) were normalized to the averaged intensity in the Ctrl-GFP-nls cells (lime-green open circles) and plotted against the length of the cell–cell junctions. n = 158 and 120 (Ctrl), 202 and 134 ( Tric -KO), 160 and 139 ( Ocln -KO), and 209 and 136 ( Tric / Ocln -dKO). *** p < 0.001 in Welch’s t test of junction-length-weighted average of the fluorescence intensity.
Rabbit Anti–Claudin 2 Pab (#51 6100), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti–claudin-2 pab (#51-6100)/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
rabbit anti–claudin-2 pab (#51-6100) - by Bioz Stars, 2026-03
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anti zo 1 pab  (Thermo Fisher)
86
Thermo Fisher anti zo 1 pab
Establishment of Tric -KO, Ocln -KO, and Tric / Ocln -dKO cells. (A, B) Ctrl, Tric -KO #1, and Tric / Ocln -dKO #1 cells (A) and Ctrl, Ocln -KO #1, and Tric / Ocln -dKO #1 cells (B) were immunostained with rat anti-tricellulin mAb (A) or rat anti-occludin mAb (B) (green) and rabbit <t>anti-ZO-1</t> <t>pAb</t> (red). All cells were stained with DAPI (blue). Note that tricellulin signal (white arrowheads) is absent in the Tric -KO and Tric / Ocln -dKO cells (magenta arrowheads) in A. Scale bars, 20 µm. (C) Immunoblotting of the total cell lysates of Ctrl, Tric -KO, Ocln -KO, and Tric / Ocln -dKO cells using rabbit anti-tricellulin mAb and rabbit anti-occludin pAb. Asterisks on the occludin panel indicate nonspecific bands. β-Actin served as a loading control.
Anti Zo 1 Pab, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti zo 1 pab/product/Thermo Fisher
Average 86 stars, based on 1 article reviews
anti zo 1 pab - by Bioz Stars, 2026-03
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alexa fluor 488 phalloidin a12379  (Thermo Fisher)
86
Thermo Fisher alexa fluor 488 phalloidin a12379
Establishment of Tric -KO, Ocln -KO, and Tric / Ocln -dKO cells. (A, B) Ctrl, Tric -KO #1, and Tric / Ocln -dKO #1 cells (A) and Ctrl, Ocln -KO #1, and Tric / Ocln -dKO #1 cells (B) were immunostained with rat anti-tricellulin mAb (A) or rat anti-occludin mAb (B) (green) and rabbit <t>anti-ZO-1</t> <t>pAb</t> (red). All cells were stained with DAPI (blue). Note that tricellulin signal (white arrowheads) is absent in the Tric -KO and Tric / Ocln -dKO cells (magenta arrowheads) in A. Scale bars, 20 µm. (C) Immunoblotting of the total cell lysates of Ctrl, Tric -KO, Ocln -KO, and Tric / Ocln -dKO cells using rabbit anti-tricellulin mAb and rabbit anti-occludin pAb. Asterisks on the occludin panel indicate nonspecific bands. β-Actin served as a loading control.
Alexa Fluor 488 Phalloidin A12379, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/alexa fluor 488 phalloidin a12379/product/Thermo Fisher
Average 86 stars, based on 1 article reviews
alexa fluor 488 phalloidin a12379 - by Bioz Stars, 2026-03
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mouse anti-ha mab  (Covance)
90
Covance mouse anti-ha mab
Establishment of Tric -KO, Ocln -KO, and Tric / Ocln -dKO cells. (A, B) Ctrl, Tric -KO #1, and Tric / Ocln -dKO #1 cells (A) and Ctrl, Ocln -KO #1, and Tric / Ocln -dKO #1 cells (B) were immunostained with rat anti-tricellulin mAb (A) or rat anti-occludin mAb (B) (green) and rabbit <t>anti-ZO-1</t> <t>pAb</t> (red). All cells were stained with DAPI (blue). Note that tricellulin signal (white arrowheads) is absent in the Tric -KO and Tric / Ocln -dKO cells (magenta arrowheads) in A. Scale bars, 20 µm. (C) Immunoblotting of the total cell lysates of Ctrl, Tric -KO, Ocln -KO, and Tric / Ocln -dKO cells using rabbit anti-tricellulin mAb and rabbit anti-occludin pAb. Asterisks on the occludin panel indicate nonspecific bands. β-Actin served as a loading control.
Mouse Anti Ha Mab, supplied by Covance, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse anti-ha mab - by Bioz Stars, 2026-03
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Image Search Results


TJs of Tric -KO, Ocln -KO, and Tric / Ocln -dKO cells appear normal in fluorescence microscopy. (A–D) Ctrl, Tric -KO #1, Ocln -KO #1, or Tric / Ocln -dKO #1 cells were mixed and cocultured with Ctrl-GFP-nls cells (green). Cells were immunostained with rat anti-ZO-1 mAb (A), mouse anti-cingulin mAb (B), mouse anti–claudin-1 mAb (C) or rabbit anti–claudin-3 pAb (D) (red) and stained with DAPI (blue). Scale bars, 20 µm. (E) Quantification of fluorescence intensity of claudin-3 at cell–cell junctions. The intensity at each cell–cell junction in the Ctrl (dark blue closed circles), Tric -KO #1 (orange closed circles), Ocln -KO #1 (green closed circles), and Tric / Ocln -dKO #1 cells (magenta closed circles) were normalized to the averaged intensity in the Ctrl-GFP-nls cells (lime-green open circles) and plotted against the length of the cell–cell junctions. n = 158 and 120 (Ctrl), 202 and 134 ( Tric -KO), 160 and 139 ( Ocln -KO), and 209 and 136 ( Tric / Ocln -dKO). *** p < 0.001 in Welch’s t test of junction-length-weighted average of the fluorescence intensity.

Journal: Molecular Biology of the Cell

Article Title: Occludin and tricellulin facilitate formation of anastomosing tight-junction strand network to improve barrier function

doi: 10.1091/mbc.E20-07-0464

Figure Lengend Snippet: TJs of Tric -KO, Ocln -KO, and Tric / Ocln -dKO cells appear normal in fluorescence microscopy. (A–D) Ctrl, Tric -KO #1, Ocln -KO #1, or Tric / Ocln -dKO #1 cells were mixed and cocultured with Ctrl-GFP-nls cells (green). Cells were immunostained with rat anti-ZO-1 mAb (A), mouse anti-cingulin mAb (B), mouse anti–claudin-1 mAb (C) or rabbit anti–claudin-3 pAb (D) (red) and stained with DAPI (blue). Scale bars, 20 µm. (E) Quantification of fluorescence intensity of claudin-3 at cell–cell junctions. The intensity at each cell–cell junction in the Ctrl (dark blue closed circles), Tric -KO #1 (orange closed circles), Ocln -KO #1 (green closed circles), and Tric / Ocln -dKO #1 cells (magenta closed circles) were normalized to the averaged intensity in the Ctrl-GFP-nls cells (lime-green open circles) and plotted against the length of the cell–cell junctions. n = 158 and 120 (Ctrl), 202 and 134 ( Tric -KO), 160 and 139 ( Ocln -KO), and 209 and 136 ( Tric / Ocln -dKO). *** p < 0.001 in Welch’s t test of junction-length-weighted average of the fluorescence intensity.

Article Snippet: Rabbit anti–ZO-1 pAb (#61-7300), rabbit anti-tricellulin mAb (clone 54H19L38; #700191), rabbit anti–claudin-2 pAb (#51-6100), rabbit anti–claudin-3 pAb (#34-1700), and mouse anti–claudin-4 mAb (#32-9400) were from Thermo Fisher Scientific (USA).

Techniques: Fluorescence, Microscopy, Staining

Establishment of Tric -KO, Ocln -KO, and Tric / Ocln -dKO cells. (A, B) Ctrl, Tric -KO #1, and Tric / Ocln -dKO #1 cells (A) and Ctrl, Ocln -KO #1, and Tric / Ocln -dKO #1 cells (B) were immunostained with rat anti-tricellulin mAb (A) or rat anti-occludin mAb (B) (green) and rabbit anti-ZO-1 pAb (red). All cells were stained with DAPI (blue). Note that tricellulin signal (white arrowheads) is absent in the Tric -KO and Tric / Ocln -dKO cells (magenta arrowheads) in A. Scale bars, 20 µm. (C) Immunoblotting of the total cell lysates of Ctrl, Tric -KO, Ocln -KO, and Tric / Ocln -dKO cells using rabbit anti-tricellulin mAb and rabbit anti-occludin pAb. Asterisks on the occludin panel indicate nonspecific bands. β-Actin served as a loading control.

Journal: Molecular Biology of the Cell

Article Title: Occludin and tricellulin facilitate formation of anastomosing tight-junction strand network to improve barrier function

doi: 10.1091/mbc.E20-07-0464

Figure Lengend Snippet: Establishment of Tric -KO, Ocln -KO, and Tric / Ocln -dKO cells. (A, B) Ctrl, Tric -KO #1, and Tric / Ocln -dKO #1 cells (A) and Ctrl, Ocln -KO #1, and Tric / Ocln -dKO #1 cells (B) were immunostained with rat anti-tricellulin mAb (A) or rat anti-occludin mAb (B) (green) and rabbit anti-ZO-1 pAb (red). All cells were stained with DAPI (blue). Note that tricellulin signal (white arrowheads) is absent in the Tric -KO and Tric / Ocln -dKO cells (magenta arrowheads) in A. Scale bars, 20 µm. (C) Immunoblotting of the total cell lysates of Ctrl, Tric -KO, Ocln -KO, and Tric / Ocln -dKO cells using rabbit anti-tricellulin mAb and rabbit anti-occludin pAb. Asterisks on the occludin panel indicate nonspecific bands. β-Actin served as a loading control.

Article Snippet: Rabbit anti–ZO-1 pAb (#61-7300), rabbit anti-tricellulin mAb (clone 54H19L38; #700191), rabbit anti–claudin-2 pAb (#51-6100), rabbit anti–claudin-3 pAb (#34-1700), and mouse anti–claudin-4 mAb (#32-9400) were from Thermo Fisher Scientific (USA).

Techniques: Staining, Western Blot

TJs of Tric -KO, Ocln -KO, and Tric / Ocln -dKO cells appear normal in fluorescence microscopy. (A–D) Ctrl, Tric -KO #1, Ocln -KO #1, or Tric / Ocln -dKO #1 cells were mixed and cocultured with Ctrl-GFP-nls cells (green). Cells were immunostained with rat anti-ZO-1 mAb (A), mouse anti-cingulin mAb (B), mouse anti–claudin-1 mAb (C) or rabbit anti–claudin-3 pAb (D) (red) and stained with DAPI (blue). Scale bars, 20 µm. (E) Quantification of fluorescence intensity of claudin-3 at cell–cell junctions. The intensity at each cell–cell junction in the Ctrl (dark blue closed circles), Tric -KO #1 (orange closed circles), Ocln -KO #1 (green closed circles), and Tric / Ocln -dKO #1 cells (magenta closed circles) were normalized to the averaged intensity in the Ctrl-GFP-nls cells (lime-green open circles) and plotted against the length of the cell–cell junctions. n = 158 and 120 (Ctrl), 202 and 134 ( Tric -KO), 160 and 139 ( Ocln -KO), and 209 and 136 ( Tric / Ocln -dKO). *** p < 0.001 in Welch’s t test of junction-length-weighted average of the fluorescence intensity.

Journal: Molecular Biology of the Cell

Article Title: Occludin and tricellulin facilitate formation of anastomosing tight-junction strand network to improve barrier function

doi: 10.1091/mbc.E20-07-0464

Figure Lengend Snippet: TJs of Tric -KO, Ocln -KO, and Tric / Ocln -dKO cells appear normal in fluorescence microscopy. (A–D) Ctrl, Tric -KO #1, Ocln -KO #1, or Tric / Ocln -dKO #1 cells were mixed and cocultured with Ctrl-GFP-nls cells (green). Cells were immunostained with rat anti-ZO-1 mAb (A), mouse anti-cingulin mAb (B), mouse anti–claudin-1 mAb (C) or rabbit anti–claudin-3 pAb (D) (red) and stained with DAPI (blue). Scale bars, 20 µm. (E) Quantification of fluorescence intensity of claudin-3 at cell–cell junctions. The intensity at each cell–cell junction in the Ctrl (dark blue closed circles), Tric -KO #1 (orange closed circles), Ocln -KO #1 (green closed circles), and Tric / Ocln -dKO #1 cells (magenta closed circles) were normalized to the averaged intensity in the Ctrl-GFP-nls cells (lime-green open circles) and plotted against the length of the cell–cell junctions. n = 158 and 120 (Ctrl), 202 and 134 ( Tric -KO), 160 and 139 ( Ocln -KO), and 209 and 136 ( Tric / Ocln -dKO). *** p < 0.001 in Welch’s t test of junction-length-weighted average of the fluorescence intensity.

Article Snippet: Rabbit anti–ZO-1 pAb (#61-7300), rabbit anti-tricellulin mAb (clone 54H19L38; #700191), rabbit anti–claudin-2 pAb (#51-6100), rabbit anti–claudin-3 pAb (#34-1700), and mouse anti–claudin-4 mAb (#32-9400) were from Thermo Fisher Scientific (USA).

Techniques: Fluorescence, Microscopy, Staining